Synthesis and identification of penicillic-acid antigens from Penicillium cyclopium / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 898-902, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-342819
ABSTRACT
To establish a new immune assay for Penicillic Acid (PA) from Penicillium cyclopium, we studied the synthesis of conjugated complete antigens for penicillic acid. PA was conjugated to bovine serum album (BSA) and ovalbumin (OVA) by 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide hydrochloride (EDC). The artificial antigens PA-BSA and PA-OVA were identified by ultraviolet spectrometric scanning, SDS-PAGE and immunization. Results showed that the absorption peak of conjugation were different from that of the carrier protein alone and of the PA. The conjugated ratio of PA and BSA was 23.21 and that of PA and OVA was 10.41. Balb/c mice were immunized by the artificial antigen of PA-BSA, with PA-OVA as coating antigen. The average titer of antiserums was more than 12 800 by indirect ELISA. The obtained antigens offered a basis for developing immunoassay method.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ácido Penicílico
/
Penicillium
/
Sangue
/
Soroalbumina Bovina
/
Ensaio de Imunoadsorção Enzimática
/
Química
/
Ovalbumina
/
Imunização
/
Alergia e Imunologia
/
Etildimetilaminopropil Carbodi-Imida
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS