Sequential expression of Wnt/beta-catenin signal pathway related genes and adipocyte transcription factors during porcine adipose tissue development / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 746-753, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-342841
ABSTRACT
To evaluate the influence of Wnt/beta-catenin signal pathway on the porcine adipose tissue development and explore the mechanism, we detected the mRNA expression of Wnt/beta-catenin signal pathway related genes beta-catenin, GSK3beta, Fzl and adipogenic transcription factors PPARy, C/EBPalpha and early differentiation marker gene LPL with semi-quantitative (SQ) RT-PCR method. Immunohistochemical method (IHC) was applied to qualitatively measure the sequential expression of beta-catenin protein. The results of SQ RT-PCR showed that beta-catenin highly expressed at the first day after birth, then decreased to a low plateau after 60 days, the expression of GSK3beta and Fzl also decreased with the development process of the porcine adipose tissue development. However, the sequential expression of PPARgamma, C/EBPalpha, LPL appeared to be an opposite manner and kept at a high level after 60 days. The result of IHC showed that the expression of beta-catenin protein was strong in nucleus and cytoplasm at the first day after birth, then tended to decline with the process of adipose tissue development and could be only found in cytoplasm after 30-day old. These results suggest that beta-catenin plays an important role in the undifferentiated state maintenance of preadipocytes and the inhibition of porcine adipose tissue development, the mechanism maybe due to its regulation function on the adipogenic transcription factors PPARy, C/EBPalpha and early differentiation marker gene LPL.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
Fatores de Transcrição
/
RNA Mensageiro
/
Transdução de Sinais
/
Tecido Adiposo
/
Adipócitos
/
Regulação da Expressão Gênica no Desenvolvimento
/
Proteínas Wnt
/
Beta Catenina
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS