Rapid detection of Pseudomonas aernginosa by the fluorescence quantitative TaqMan PCR assay targetting ETA gene / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 581-585, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-342867
ABSTRACT
Pseudomonas aernginosa (PA) is one of the most universal pathogens in clinical diagnosis, and conventional detection assay has many disadvantages. In this research, a pair of specific primers and a TaqMan fluorescent probe were designed in the conservative region of ETA gene by the method of bioinformatics analysis, the detection method for PA was successfully developed. Different gradient concentrations of PA DNA and various pathogen DNA were amplified by fluorescence quantitative PCR (FQ-PCR) to confirm the specificity and sensitivity of the developed method. Results showed that the developed detection assay is more sensible and specific by comparison to the conventional FQ-PCR method, and it is valuable for research and application prospects.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pseudomonas aeruginosa
/
Toxinas Bacterianas
/
DNA Bacteriano
/
Reação em Cadeia da Polimerase
/
Sensibilidade e Especificidade
/
ADP Ribose Transferases
/
Taq Polimerase
/
Fatores de Virulência
/
Exotoxinas
/
Corantes Fluorescentes
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS