Secretory expression and biological function of IGFBP-3 in eukaryotic cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 558-562, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-342871
ABSTRACT
The cDNA of Insulin-like growth factor binding protein 3 was subcloned into a eukaryotic secretory expression vector pSectagA to construct pSectag-IGFBP3. Human renal cell carcinoma (RCC) 786-0 cells were transfected with pSectag-IGFBP3 using lipofectamine 2000. After 48 h, the secretory IGFBP-3 was tested and identified by western blotting. Meanwhile, Annexin V-EGFP stain was used to analyze the apoptosis of 786-0 cells induced by IGFBP-3. Secretory IGFBP-3 protein could express successfully in the 786-0 cells and the expressed IGFBP-3 directly displayed an apoptotic effect on the host cells. This work provides a basis for further study on the apoptosis-inducing mechanism of IGFBP-3 and the development of a new anti-tumor drug.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Fisiologia
/
Proteínas Recombinantes de Fusão
/
Dados de Sequência Molecular
/
Células Tumorais Cultivadas
/
Sequência de Bases
/
Carcinoma de Células Renais
/
Transfecção
/
Apoptose
/
Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2008
Tipo de documento:
Artigo
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