The influences of cross-linking agent on the composite bio-sponge / 中国医疗器械杂志
Chinese Journal of Medical Instrumentation
;
(6): 317-320, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-342939
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of cross-linking agents genipin and glutaraldehyde on the composite bio-sponge.</p><p><b>METHODS</b>The composite bio-sponge was prepared with the technology of lyophilization. The degree of cross linking was determined using absorptiometry of trinitrobenzenesulfonic acid; the cytotoxicity was tested by MTT assay; the degradation rate in vitro was valuated by lysozyme degradation.</p><p><b>RESULTS</b>(1) The degree of cross linking of composite bio-sponge crosslinked using genipin and glutaraldehyde increased with the crosslinking time, and reached 26.43% and 54.63% respectively after crosslinking 3 d. (2) The water absorption rate of composite bio-sponge crosslinked using genipin was better than that of crosslinked using glutaraldehyde. (3) In the initial stage of cells incubation, all extracts of composite bio-sponges crosslinked using genipin and glutaraldehyde inhibited the growth of the cells, and the inhibition decreased with the incubation time; but the cytotoxicity of composite bio-sponge crosslinked using glutaraldehyde was higher than that of crosslinked using genipin. (4) After soaking in saline for 4 weeks, the degradation rate of composite bio-sponge crosslinked using genipin or glutaraldehyde was 32.1%, 28.4%, respectively; however, after soaking in saline containing lysozyme for 40 h, the degradation rate of composite bio-sponge was 36.7%, 31.2%, respectively.</p><p><b>CONCLUSION</b>Compared with the composite bio-sponge crosslinked using glutaraldehyde, the degree of cross linking and the cytotoxicity of the composite bio-sponge crosslinked using genipin decreased; however, the water absorption rate and the degradation rate increased.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Materiais Biocompatíveis
/
Teste de Materiais
/
Química
/
Glutaral
/
Reagentes de Ligações Cruzadas
/
Iridoides
Idioma:
Chinês
Revista:
Chinese Journal of Medical Instrumentation
Ano de publicação:
2012
Tipo de documento:
Artigo
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