Protective effect of mouse 2.5s nerve growth factor on PC12 cells from injury induced by 2, 5-hexanedione / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 154-156, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-343032
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether the nerve growth factor has protective effects on PC12 cells from injury induced by 2, 5-hexanedione.</p><p><b>METHODS</b>With PC12 cells as the model of neurons, different concentrations of NGF were added into the culture of PC12 cells. Then cell viability was tested with MTT. The DNA fragment was observed with agarose gel electrophoresis. The apoptosis ratio was tested with flow cytometry (FACS). The p53 protein was detected with western blot. The differences among the groups were compared.</p><p><b>RESULTS</b>Cell viabilities were increased with the increase of the concentrations of NGF (P < 0.05). The DNA fragment, the apoptosis ratio and the expression of p53 were all decreased with the increase of the concentrations of NGF (P < 0.05).</p><p><b>CONCLUSION</b>The NGF might have direct nutritional effects on PC12 cells, and protect them from injury induced by 2, 5 HD. Moreover, it might also have anti-apoptosis effect to some extent.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Proteína Supressora de Tumor p53
/
Células PC12
/
Apoptose
/
Relação Dose-Resposta a Droga
/
Eletroforese em Gel de Ágar
/
Toxicidade
/
Fragmentação do DNA
/
Citometria de Fluxo
/
Hexanonas
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2006
Tipo de documento:
Artigo
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