Decreased Expression of 15-hydroxyprostaglandin Dehydrogenase in Gastric Carcinomas
Yonsei Medical Journal
;
: 917-922, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-34313
ABSTRACT
PURPOSE:
Gastric carcinoma tissues release high level of prostaglandin E2 (PGE2) when compared to non-neoplastic mucosa, and cyclooxygenase-2 (COX-2), which is the rate-limiting enzyme in prostaglandin (PG) biosynthesis, is often overexpressed in gastric carcinomas and during gastric carcinogenesis. However, little is known about the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), the key enzyme responsible for the biological inactivation of PG, in gastric carcinomas. MATERIALS ANDMETHODS:
We investigated the expression of 15-PGDH in 28 cases of advanced gastric carcinomas by Western blot analysis and also the relation between its expression and the gene promoter methylation.RESULTS:
15-PGDH expression was significantly decreased in gastric carcinomas compared to corresponding non-neoplastic tissues and inversely correlated with the expression of proliferating cell nuclear antigen in gastric carcinomas. However, there was no correlation between 15-PGDH expression and pathological findings such as nodal metastasis and vascular invasion. Promoter hypermethylation of 15-PGDH gene was not detected in carcinomas, with only a negligible expression of the enzyme.CONCLUSION:
Our results suggested that 15-PGDH has tumor suppressor activity in gastric carcinomas.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Gástricas
/
DNA de Neoplasias
/
Sequência de Bases
/
Hidroxiprostaglandina Desidrogenases
/
Regiões Promotoras Genéticas
/
Primers do DNA
/
Metilação de DNA
Limite:
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2008
Tipo de documento:
Artigo
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