Investigation of uninterpretative HLA typing in 311 umbilical cord blood samples / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1261-1264, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-343306
ABSTRACT
The aim of this study was to investigate the factors which affect HLA typing in 311 umbilical cord blood (UCB) samples. The HLA low resolution typing of UCB samples with misinterpreted HLA types from 311 UCB samples analyzed by PCR-SSO and PCR-SSP was performed. 7 samples difficult to determine their HLA genotype were sequenced directly and the reason leading to misinterpret HLA typing was analyzed. The results indicated that 99.4% of misinterpreted samples resulted from the restriction of HLA typing method itself and 0.6% of misinterpreted samples were suspected to be contaminated with maternal blood in UCB. It is concluded that HLA typing is mainly affected by the shortcomings of oligonucleotide probe design for PCR-SSO and lack of allele specific primers of PCR-SSP.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Teste de Histocompatibilidade
/
Sequência de Bases
/
Sondas de Oligonucleotídeos
/
Reação em Cadeia da Polimerase
/
Primers do DNA
/
Alelos
/
Alergia e Imunologia
/
Sangue Fetal
/
Genética
/
Genótipo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2009
Tipo de documento:
Artigo
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