Changes and mechanism of apoptosis-related gene expression in T lymphocytic leukemia JM cells induced with matrine / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1207-1210, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-343318
ABSTRACT
This study was purposed to investigate the changes of apoptosis-related gene expression in T lymphocytic leukemia JM cells induced with matrine, and its possible mechanism. JM cells was induced with 0.4 mg/ml matrine for 4 days, the total RNA was extracted from JM cells before and after matrine induction, the differential expression of apoptosis-related genes were screened with cDNA Expression Array Kit, the expression change of a part of gene was checked by Western blot. The results indicated that after induction of JM cells with matrine, differential expression of 31 genes were found by gene chip hybridization, the expression of caspase 8 was up-regulated more than 5 times. Western blot analysis showed that the up-regulation of caspase 8 gene expression positively correlated with induction time. It is concluded that differential expressions of many apoptosis-related genes in JM cells can be induced by matrine, in which gene expression of caspase 8 is up-regulated notably.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Quinolizinas
/
Leucemia
/
Regulação Neoplásica da Expressão Gênica
/
Regulação para Cima
/
Apoptose
/
Linhagem Celular Tumoral
/
Alcaloides
/
Caspase 8
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2009
Tipo de documento:
Artigo
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