Activated T cells promote differentiation of mesenchymal stem cells into osteoblasts / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 974-976, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-343368
ABSTRACT
This study was aimed to investigate the effect of activated T cell on the ability of MSC to differentiate into osteoblasts. The activated T cells with MSCs were co-culture for 14 days, then the osteoblast formation was tested by alkaline phosphatase staining. Furthermore, the supernatant of activated T cell was added in culture system of MSCs, the expression of molecules related with immune regulation of activated T cells was detected by RT-PCR, so as to determine what kinds of cytokine displayed the important function in MSC differentiation. The result showed that activated T cell could promote differentiation of MSC into osteoblasts, and IL-1beta played an important role in the effect of activated T cells on MSCs, while TNF-alpha, TGF-beta1 were not. It is concluded that the activated T cells promote the differentiation of MSCs to osteoblasts. The interactive influence between MSCs and immune cells can be mediated through cytokines.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Linfócitos T
/
Diferenciação Celular
/
Células Cultivadas
/
Meios de Cultivo Condicionados
/
Técnicas de Cocultura
/
Biologia Celular
/
Interleucina-1beta
/
Células-Tronco Mesenquimais
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2009
Tipo de documento:
Artigo
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