Construction of pGEX4T-1-Cox7a2 and expression, purification and identification of the recombinant protein / 中华男科学杂志
National Journal of Andrology
;
(12): 794-797, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-343521
ABSTRACT
<p><b>OBJECTIVE</b>To clone and express Cox7a2, one mitochondrial respiratory chain related gene, and to identify its recombinant protein.</p><p><b>METHODS</b>The coding region of Cox7a2 was amplified from primary cultured mouse Leydig cells by RT-PCR. The PCR product was cloned into pGEX4T-1 vector by BamH I and EcoR I sites, and confirmed by DNA sequencing. The recombinant fusion protein vector was transformed and expressed into BL21. The recombinant fusion protein was identified by Western blotting.</p><p><b>RESULTS</b>The entire coding region of Cox7a2 was cloned and expressed. The fusion protein was identified by anti-GST monoclonal antibody using Western blotting.</p><p><b>CONCLUSION</b>The cloning of Cox7a2 and the expression of the recombinant protein would help to study the detailed function of Cox7a2, one respiratory chain related and highly differently expressed gene in the tissues of aging testes.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Proteínas Recombinantes de Fusão
/
Linhagem Celular
/
Clonagem Molecular
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vetores Genéticos
/
Genética
/
Células Intersticiais do Testículo
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS