Expression of multi-copy and immuno-reactivity of recombinant type-specific epitope of herpes simplex virus type 1 / 中华男科学杂志
National Journal of Andrology
;
(12): 579-582, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-343570
ABSTRACT
<p><b>OBJECTIVE</b>To express a multi-copy specific epitope recombinant protein of herpes simplex virus type 1 (HSV-1) with immuno-reactivity.</p><p><b>METHODS</b>Multi-copy genes with a specific epitope of HSV-1-glycoprotein G 112-127 were constructed by DNA recombination and cloned in E. coli JM109 pGEM-5Zf. The positive recombinants were determined by SDS-PAGE and Western blotting.</p><p><b>RESULTS</b>The recombinants with 4, 8, 16 and 32 copies of gG 112-127 were obtained. The 8-copy recombinant was expressed by 17.5%, mainly as inclusion body. And it reacted with antiserum HSV-1, but not with antiserum HSV-2.</p><p><b>CONCLUSION</b>The HSV-1-gG112-127 recombinant could be used to distinguish HSV-1 and HSV-2 in ELISA.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes de Fusão
/
DNA Recombinante
/
Proteínas do Envelope Viral
/
Herpesvirus Humano 2
/
Herpesvirus Humano 1
/
Alergia e Imunologia
/
Reações Antígeno-Anticorpo
/
Epitopos
Limite:
Humanos
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2006
Tipo de documento:
Artigo
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