Effect of aluminum trichloride on abnormal phosphorylation of tau protein in SH-SY5Y cells / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 100-103, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-343697
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of aluminum trichloride on the abnormal phosphorylation of tau protein in SH-SY5Y cells.</p><p><b>METHODS</b>SH-SY5Y cells were assigned to control group and aluminum trichloride exposure groups (200, 400, and 800 µmol/L Al(3+)). The cell morphology was observed after 48 hours of exposure; the cell viability was measured by CCK-8 assay; total protein was extracted from the cells, and the expression of phospho-tau (p-tau) 181, 231, 262, and 396 and tau 5 was measured by Western blot.</p><p><b>RESULTS</b>As the Al(3+) concentration rose, the number of living SH-SY5Y cells decreased, and the synapses of the cells retracted. The viability of cells exposed to 800 µmol/L Al(3+) was significantly lower than that of the control group (P < 0.05). The 200, 400, and 800 µmol/L Al(3+) exposure groups showed significantly higher expression of p-tau 181, 231, and 396 and tau5 than the control group (P < 0.05), and the 800 µmol/L Al(3+) exposure group showed significantly higher expression of p-tau 262 than the control group (P < 0.05).</p><p><b>CONCLUSION</b>Under the present experimental conditions, aluminum trichloride has toxic effect on SH-SY5Y cells and can lead to abnormal expression of p-tau 181, 231, and 396 and tau 5 at low Al(3+) concentration.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Sobrevivência Celular
/
Cloretos
/
Proteínas tau
/
Compostos de Alumínio
/
Linhagem Celular Tumoral
/
Toxicidade
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2013
Tipo de documento:
Artigo
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