Effect of tetanus toxoid powder needleless injection on the concentration of serum antibody IgG in mice / 药学学报
Acta Pharmaceutica Sinica
; (12): 1406-1409, 2009.
Article
em Zh
| WPRIM
| ID: wpr-344062
Biblioteca responsável:
WPRO
ABSTRACT
In this study, a self-designed powder needleless injection system was compared with subcutaneous injection using a needle and syringe to deliver tetanus toxoid (TT) into mice to elicit immunity. First of all, factors influencing the prepartion of TT into powder by being absorbed on aluminium hydroxide were investigated and the micromeritic characters of Al (OH)3-TT powder were observed with optical microscope and laser particle analyzer. The results showed that salt concentration and absorption time had an enhancive effect on drug loading, but the pH value and temperature did not influence the absorption reaction obviously. The absorption reaction was optimized with sodium chloride concentration of 0.4 mol x L(-1) and lasting for 10 min. The average diameter of Al(OH)3-TT powder prepared with conditions optimized above was (60.6 +/- 4.4) microm. The immunization effect of TT was determined through enzyme-linked immunosorbent assay (ELISA) of the concentration of IgG antibody elicited by TT. With delivery of Al(OH)3-TT (of 30 microg TT) by powder needleless injection to mice, the IgG antibody concentration were (6.19 +/- 0.52) and (10.70 +/- 0.78) U x L(-1) after immunization of 4 and 8 weeks, respectively, while the values were (4.25 +/- 0.58) and (7.48 +/- 0.57) U x L(-1) by subcutaneous injection (of 20 microg TT) using a needle and syringe. The results suggested that the self-designed powder needleless injection of Al(OH)3-TT was comparable to subcutaneous injection with a good immunity.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Tamanho da Partícula
/
Pós
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Sangue
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Imunoglobulina G
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Injeções a Jato
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Toxoide Tetânico
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Adjuvantes Imunológicos
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Composição de Medicamentos
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Alergia e Imunologia
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Hidróxido de Alumínio
Limite:
Animals
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2009
Tipo de documento:
Article