Establishment and characteristics of acute lung injury model induced by cigarette smoke in ICR mice / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To develop a mouse model of acute lung injury induced by cigarette smoke (CS) and to investigate inflammatory changes with the model.</p><p><b>METHODS</b>ICR mice exposed to CS for 20-min, 3/d. Bronchoalveolar lavage fluid (BALF) and lung tissue were harvested at d 0, d 1, d 3 and d 7 after CS exposure. Neutrophil count in BAFL, TNF-alpha and MMP-12 levels, the activity of MPO in lung tissue were determined.</p><p><b>RESULT</b>Neutrophil count in BALF, MMP-12 and MPO levels in lung tissue were increased after CS exposure in a time-dependent manner with a peak at d3. TNF-alpha level sharply increased at d1, and remained high level until d7.</p><p><b>CONCLUSION</b>ICR mice are tolerant and sensitive to CS exposure, which may be used as an appropriate animal model for acute lung injury induced by cigarette smoke.</p>