Luteolin excretion after oral administration of Elsholtzia blanda benth extracts in rats / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To observe the excretion of luteolin after oral administration of Elsholtzia blanda benth extracts in rats.</p><p><b>METHODS</b>Samples of urine, feces and bile were collected after oral administration of Elsholtzia blanda benth extracts in rate. After deconjugation with beta-glucuronidase/sulfatase, the levels of luteolin in urine, feces and bile were measured by RP-HPLC.</p><p><b>RESULT</b>The recovery rate of luteolin was 98.0 %-106.0 % and the extract recoveries were 85.0 %-108.0%. Relative standard deviation (RSD) of intra-and inter-day assay was less than 10.0 %. The total accumulative excretion was 37 % (11 % in urine, 26 % in feces and bile).</p><p><b>CONCLUSION</b>The established RP-HPLC method is sensitive, specific, accurate, and is applicable for determination of luteolin in rat urine,feces and bile.</p>