Quasispecies and mutation of hepatitis B virus polymerase gene in lamivudine- treated patients / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 235-238, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-344442
ABSTRACT
<p><b>OBJECTIVE</b>To study the quasispecies and mutation features of hepatitis B virus polymerase (HBV P) gene in chronic hepatitis B patients before and after lamivudine treatment.</p><p><b>METHODS</b>The HBV P gene was amplificated with PCR and cloned, then single strand conformation polymorphism / heteroduplex analysis (SSCP/HDA) was applied to analyze the quasispecies complexity and mutation characters of HBV P gene.</p><p><b>RESULTS</b>The quasispecies number of HBV P gene before treatment was larger than that after treatment (7 to 14 vs. 4 to 8, t = 3.98, P < 0.05). Six patients had one or two predominant quasispecies before therapy, but the percentages of predominant quasispecies were lower than those after therapy (33.3% to 81.8% vs. 78.8% to 90.9%, t = 3.42, P < 0.05). By sequencing the predominant clones, there were 2 patients with M550V/L528M mutation, 3 patients with M550I mutation and 1 patient with wild type after lamivudine therapy. Additionally, there were individualization mutations which had no obvious tender.</p><p><b>CONCLUSION</b>Quasispecies of hepatitis B virus are changed under the selection of lamivudine, meanwhile, the mutation at YMDD motif emerges.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Antivirais
/
Virologia
/
DNA Viral
/
Vírus da Hepatite B
/
Classificação
/
Mutação Puntual
/
Lamivudina
/
Hepatite B Crônica
/
Usos Terapêuticos
/
DNA Polimerase Dirigida por DNA
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Artigo
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