Preliminary study on hepatitis B virus quasispecies in a patient with chronic hepatitis B / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 219-221, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-344448
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether the hepatitis B virus (HBV) has quasispecies character by studying nucleotide sequence polymorphism and mutation features of HBV PreC/C gene region, and preliminaryly explore the heterogeneity of HBV quasispecies.</p><p><b>METHODS</b>The serum sample was obtained from a patient with chronic hepatitis B, and the whole HBV PreC/C gene region was amplified by PCR and cloned. Thirty-four clones that contained HBV PreC/C gene fragments were sequenced.</p><p><b>RESULTS</b>There were 28 kinds of different nucleotide sequences in 34 clones, and the nucleotide sequences diversity ranged from 0.2% to 2.1%. The mutation points were almost distributed in the whole region, but there wasn't mutation at PreC region nt.1 896 point in all sequences.</p><p><b>CONCLUSION</b>Hepatitis B virus has complex quasispecies character in the patients with chronic hepatitis B.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Vírus da Hepatite B
/
Regiões Promotoras Genéticas
/
Classificação
/
Análise de Sequência de DNA
/
Hepatite B Crônica
/
Alergia e Imunologia
/
Genética
/
Antígenos do Núcleo do Vírus da Hepatite B
/
Mutação
Limite:
Adulto
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Artigo
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