Protecting effects of p44/42 MAPK signal transduction pathway on hepatocytes in ischemic preconditioning / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 166-169, 2003.
Article
em Zh
| WPRIM
| ID: wpr-344474
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ABSTRACT
<p><b>OBJECTIVE</b>To investigate the significance of PKC and p44/42 mitogen-activated protein kinase (MAPK) signal transduction in ischemic preconditioning (IP).</p><p><b>METHODS</b>Through liver cell IP models, PKC inhibitor and MEK inhibitor were utilized to analyze the phosphorylation level of p44/42 MAPK and cell viability was also observed. Rat liver IP models were established which were treated with various drugs. Then the phosphorylation level of p44/42 MAPK in vivo and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) concentrations were detected. And cellular structures were observed under light microscopy.</p><p><b>RESULTS</b>Similar results were obtained in vivo and in vitro IP models. Compared with the ischemia reperfusion (IR) group in vivo, the phosphorylation level of p44/42 MAPK was obviously increased in IP treated rats (q = 27.217, P < 0.01), and the cellular structure injured slightly. The concentrations of serum ALT and AST in IP group were significantly lower than those in IR group (281.0 U/L +/-35.6 U/L vs 762.8 U/L +/-130.5 U/L and 407.7 U/L +/-73.7 U/L vs 820.9 U/L +/-111.3 U/L, P < 0.01). However, opposite changes were found in PKC and MEK inhibited groups, when compared to IP group. The phosphorylation level of p44/42 MAPK was obviously decreased, the liver tissues injured evidently, and the concentrations of serum ALT and AST (645.61 U/L +/-90.4 U/L, 678.6 U/L +/-136.5U/L and 466.2 U/L +/-82.8 U/L, 732.9 U/L +/-91.1 U/L, respectively) were significantly greater than those in IP group.</p><p><b>CONCLUSION</b>These results suggest that p44/42 MAPK pathway plays a vital role in the protection of hepatocytes in ischemic preconditioning.</p>
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Índice:
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Assunto principal:
Fosforilação
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Fisiologia
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Aspartato Aminotransferases
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Sangue
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Proteína Quinase C
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Transdução de Sinais
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Ratos Sprague-Dawley
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Precondicionamento Isquêmico
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Proteína Quinase 1 Ativada por Mitógeno
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Hepatócitos
Limite:
Animals
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Article