Immune response of dendritic cells acquiring antigens from apoptotic cholangiocarcinoma cells induced by mitomycin / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 149-152, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-344479
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-tumor immune response of dendritic cells (DCs) acquiring antigens from apoptotic cholangiocarcinoma cells and their therapeutic effects on cholangiocarcinoma cells.</p><p><b>METHODS</b>DCs from human peripheral blood monocytes which acquired antigen capturing and processing capacity, characteristics of maturation, were established in vitro using granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Then cholangiocarcinoma cells were induced to apoptosis with mitomycin. The three groups included (1) coculture of DCs, apoptotic cancer cells and T cells, (2) coculture of DCs, necrotic cancer cells and T cells, (3) coculture of DCs, cultured cancer cells and T cells. After 7 days, DCs and T cells were riched separately to perform anti-tumor cells test and immune response test.</p><p><b>RESULTS</b>these cells had typical dendritic cell morphology, expressed high levels of CD1a and B7, acquired antigen from apoptotic cells caused by mitomycin and could stimulate T cells to inhibit, even kill cholangiocarcinoma cells.</p><p><b>CONCLUSIONS</b>The DCs from peripheral blood monocytes induced by GM-CSF and IL-4 can efficiently present antigen derived from apoptotic cells caused by mitomycin, and stimulate T cells activity obviously. It maybe become an effective therapy for tumor.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Neoplasias dos Ductos Biliares
/
Ductos Biliares Intra-Hepáticos
/
Células Dendríticas
/
Células Tumorais Cultivadas
/
Linfócitos T
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Interleucina-4
/
Mitomicina
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Artigo
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