Generation of high affinity humanized single-chain antibody against PreS1 of hepatitis B virus from immune phage displayed antibody library / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 90-92, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-344506
ABSTRACT
<p><b>OBJECTIVE</b>Screening and characterizing high affinity completely humanized single-chain antibody (ScFv) against PreS1 of hepatitis B virus.</p><p><b>METHODS</b>A combinatorial library of phage-displayed human ScFv, genes of which were derived from peripheral blood lymphocytes immunized by PreS1 of Hepatitis B Virus in vitro, was constructed. The library contained 7 10(8) clones.</p><p><b>RESULTS</b>After 3 rounds panning, a high affinity (K=10(7) to 10(8) mol/L) ScFv specific to PreS1 was obtained. The V(H) belonged to human V(H4) family, and V(1) to V(4) by sequence analysis.</p><p><b>CONCLUSION</b>This study suggests that the method of antigen stimulation in vitro is an expeditious way for the source of human immune antibody. And the ScFv may provide a more satisfactory therapy.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Precursores de Proteínas
/
Biblioteca de Peptídeos
/
Alergia e Imunologia
/
Anticorpos Anti-Hepatite B
/
Antígenos de Superfície da Hepatite B
/
Anticorpos Monoclonais
/
Afinidade de Anticorpos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2003
Tipo de documento:
Artigo
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