Synthesis and optimize reaction conditions of chlorogenic acid artificial antigen / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To synthesize artificial antigen of the chlorogenic acid (CGA), a constituent in Traditional Chinese Medicine, then optimize the reaction conditions, so that to supply the basis in material and technique for study on Chinese medicine injection of adverse reactions in-depth.</p><p><b>METHOD</b>The complete antigen of chlorogenic acid (CGA-BSA) was synthesized by CGA coupled to carrier protein bovine serum albumi (BSA) using carbodiimide method. We used the orthogonal design test to optimize reaction conditions and used the MALDI-TOF-MS and UV motheds to determine the coupling rate of the CGA-BSA.</p><p><b>RESULT</b>The coupling rate were 20 and 18.8, counting by MALDI-TOF-MS and UV, respectively; while the best reaction conditions were NHS CGA, EDC CGA, CGA BSA were 4 1, 1 1, 200 1, respectively, with the pH 9.</p><p><b>CONCLUSION</b>The complete antigen is preparated successfully. Which supplied the basis to advanced study of correlation.</p>