Agrobacterium tumefaciens mediated Chitinase and beta-1,3-glucanase gene transformation for Pinellia ternata / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To obtain transgenic Pinellia ternata plants resistant to fungus by transfer Chitinase and beta-1,3-Glucanase gene from Trichoderma harzianum.</p><p><b>METHOD</b>Using hygromycin phosphotransferase as the selection marker, the Chitinase gene (ech42), beta-1,3-Glucanase gene (gluc78) and both gene pCAMBIA(ech42 + gluc78) driven by CaMV35S promoter were transferred into P. ternata callus via Agrobacterium-mediated transformation.</p><p><b>RESULT</b>PCR results confirmed that the regenerants were identified to be transgenic lines and the RT-PCR results confirmed that foreign genes construction were transfer to mRNA. Two foreign genes were inherited stably to T5 generation according to PCR results of the lines.</p><p><b>CONCLUSION</b>The results showed that chitinase gene ech42 and beta-1, 3-glucanase gene gluc78 respectively or together introducing and co-integrating into P. ternata</p>