Accumulation of flavonoids and chlorogenic acid in callus and suspension cell of Eucommia ulmoides / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze and compare the cell growth and accumulation of flavonoids and chlorogenic acid in the callus and suspension cell of Eucommia ulmoides.</p><p><b>METHOD</b>The callus induced from the leaf of E. ulmoides seedlings were suspended in liquid medium. The time courses of cell growth and yields of flavonoids and chlorogenic acid were studied.</p><p><b>RESULT</b>The highest contents of flavonoids and chlorogenic acid in the callus were 13.46, 1.712 mg x g(-1), respectively, while the contents of these two secondary metabolites were 16.63, 3.93 mg x g(-1) in suspension cell culture correspondingly.</p><p><b>CONCLUSION</b>Comparing with callus, the suspension cell showed a short growth period and high growth rate with a remarkable high content of flavonoids and chlorogenic acid.</p>