Influence of ground beetle on steroid-induced BMSCs osteogenic differentiation / 中国骨伤
China Journal of Orthopaedics and Traumatology
;
(12): 921-924, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-344697
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the pathogenesis of steroid-induced avascular necrosis of femoral head and the control mechanisms of Ground Beetle.</p><p><b>METHODS</b>The bone marrow-derived mesenchymal stem cells were cultured in vitro. The BMSCs in the 3rd generation were randomly divided into blank group, model group and Chinese medicine low, medium and high dose group. BMSCs of model group using high-dose steroid-induced in vitro were adipogenic differentiation to inhibit osteogenesis. Chinese medicine low, medium and high-dose groups at the same time were given the Ground Beetle intervention serum containing insects. The expression of osteocalcin, Alkaline phosphatase and Type I Collagen mRNA were detected and interfered 6 days later.</p><p><b>RESULTS</b>Serum containing Ground Beetle could reverse content of steroid-induced alkaline phosphatase of BMSCs, the expression of ostercalcin and Type I Collagen mRNA decreased.</p><p><b>CONCLUSION</b>The control mechanism of Ground Beetle on steroid-induced avascular necrosis is not only to improve the microcirculation, but also to inhibit steroid-induced osteogenic differentiation of BMSCs reduced.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteogênese
/
Farmacologia
/
Besouros
/
Células da Medula Óssea
/
Dexametasona
/
Osteocalcina
/
Diferenciação Celular
/
Ratos Sprague-Dawley
/
Biologia Celular
/
Colágeno Tipo I
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Orthopaedics and Traumatology
Ano de publicação:
2010
Tipo de documento:
Artigo
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