Effects of geniposide on SNP-induced apoptosis of chondrocyte and cell cycle / 中国骨伤
China Journal of Orthopaedics and Traumatology
;
(12): 232-235, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-344752
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Geniposide on SNP(sodium nitroprusside)-induced apoptosis of chondrocyte in vitro and cell cycle.</p><p><b>METHODS</b>The chondrocyte of three-week-old SD rats were separated and cultivated. The second generation of chondrocyte cells were involved in experiment. Chondrocyte proliferation was measured by assay; flow cytometer were adopted to observe cell cycle and apoptosis rate; NO examination adopted nitrate reductase method.</p><p><b>RESULTS</b>Geniposide could significantly decrease the percentage of SNP-induced chondrocytes in G0/G1 phase and increased percentage in S phase and G2/M phase. The apoptosis of chondrocyte and the concentration of NO in the culture supernatants was reduced significantly (r=0.917, P<0.01).</p><p><b>CONCLUSION</b>Geniposide could impact SNP-induced apoptosis of chondrocyte by reducing the concentration of NO in the culture supernatants, promoting proliferation of chondrocytes, which is a probable and important mechanism of Geniposide preventing osteoarthritis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoartrite
/
Farmacologia
/
Fisiologia
/
Nitroprussiato
/
Ciclo Celular
/
Ratos Sprague-Dawley
/
Apoptose
/
Condrócitos
/
Iridoides
/
Usos Terapêuticos
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Orthopaedics and Traumatology
Ano de publicação:
2013
Tipo de documento:
Artigo
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