Influence of serum containing Liuwei Dihuang decoction (see text) on proliferation of osteoblasts under stretch-stress environment / 中国骨伤
China Journal of Orthopaedics and Traumatology
;
(12): 142-146, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-344776
ABSTRACT
<p><b>OBJECTIVE</b>To study influence of serum containing Liuwei Dihuang decoction (see text) on proliferation and differentiation of osteoblast form neonatal SD rats cultured in vitro at different times and different stretch stress.</p><p><b>METHODS</b>After osteoblast cultured for 24 hours in the serum containing Liuwei Dihuang decoction (see text) and serum in control group, the 0.5 Hz frequency, 6% and 12% stretch-stress were added. The MTT1 and the activity of ALP were measured at the 12th and 24th hours, and the data were analyzed.</p><p><b>RESULTS</b>1. In the environment of stretch stress to the frequency of 0.5 Hz, and stretched for 24 hours, the osteoblast was stimulated under elongation rate of 6% and 12%; the proliferation and differentiation of osteoblast was more active under elongation rate of 12% than that of 6%. 2. There were no stimulating effects on osteoblast proliferation and differentiation of serum containing Liuwei Dihuiang decoction (see text) acted on osteoblast cells of SD rats cultured in vitro for a shot time.</p><p><b>CONCLUSION</b>Stretch stress environment can enhance osteoblast proliferation and differentiation cultured in vitro.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Farmacologia
/
Fisiologia
/
Estresse Mecânico
/
Medicamentos de Ervas Chinesas
/
Diferenciação Celular
/
Ratos Sprague-Dawley
/
Soro
/
Proliferação de Células
/
Fosfatase Alcalina
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Orthopaedics and Traumatology
Ano de publicação:
2013
Tipo de documento:
Artigo
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