A candidate tumor suppressor gene mutated in primary hepatocellular carcinoma: kruppel-like factor 6 / 中华外科杂志
Chinese Journal of Surgery
;
(12): 1258-1261, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-345111
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between kruppel-like factor (KLF)6 gene and the development or progression of hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>Reverse-transcription polymerase chain reaction (RT-PCR) was used to examine the expression of KLF6 mRNA in normal liver tissue and primary hepatocellular carcinoma, and single strand conformation polymorphism (SSCP), DNA sequencing were used to detect the point mutation of KLF6 in primary hepatocellular carcinoma.</p><p><b>RESULTS</b>An amplified fragment of 427 bp DNA was detected in 31 (97%) of 32 adjacent noncancerous tissue and normal liver tissue, and in 23 (85%) of 27 HCCs. There was no significant difference in the levels of KLF6 mRNA between normal liver and liver tumors (chi(2) = 2.58, P > 0.05). For the 27 HCCs, six SSCP-positive bands (22%) were detected. Among them, three of 5 (3/5) tumor samples showing loss of heterozygosity (LOH) of KLF6 had mutations in the retained KLF6 allele.</p><p><b>CONCLUSION</b>We showed that LOH was detected in 5 (36%) HCCs obtained from 14 informative cases, and three of 5 tumor samples showing LOH of KLF6 had mutations in the retained KLF6 allele. Two inactivating events had occurred; thus, as defined by Knudson's "two-hit model", 16 KLF6 appears to be a tumor suppressor gene.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
RNA Mensageiro
/
Análise Mutacional de DNA
/
Análise de Sequência de RNA
/
Carcinoma Hepatocelular
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Fatores de Transcrição Kruppel-Like
/
Genética
/
Neoplasias Hepáticas
/
Mutação
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2004
Tipo de documento:
Artigo
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