Expression and clinical significance of cancer-related gene MPS-1 in gastric cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 503-506, 2005.
Article
em Zh
| WPRIM
| ID: wpr-345147
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of cancer-related gene MPS-1 in gastric cancer and to evaluate its significance in clinical diagnosis and therapy.</p><p><b>METHODS</b>The mRNA expression of MPS-1 was determined by polymerase chain reaction after reverse transcription (RT-PCR) in cancer tissues and adjacent non-cancerous tissues from 42 cases with gastric cancer. The expression levels of MPS-1 in 6 gastric cancer cell lines (AGS, MKN-45, SGC 7901, KATO III, N-87 and SNU-1) were also determined by RT-PCR and Western blot.</p><p><b>RESULTS</b>The MPS-1 mRNA was expressed in all tissues and cell lines. The mRNA expression level of MPS-1 in cancer tissues were 1.37+/- 0.87, significantly higher than 0.99+/- 0.67 in adjacent normal gastric mucous tissues (P< 0.01). The expression of MPS-1 was correlated with TNM stage (P< 0.05), but not with age, gender, tumor size and differentiation. The expression level of MPS-1 mRNA in the primary lesions was hig her in the patients with TNM stages III, IV than those with TNM stages I, II. Meanwhile, RT-PCR and Western blot showed the same results that MPS-1 expression was higher in the six gastric cancer cell lines as compared with that in the normal gastric cell line GES-1.</p><p><b>CONCLUSION</b>The high expression of MPS-1 in gastric cancer indicates that MPS-1 might play an important role in gastric carcinogenesis,which may provide a new target in immunotherapy for gastric cancer.</p>
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Índice:
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Assunto principal:
Patologia
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Neoplasias Gástricas
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Proteínas Tirosina Quinases
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RNA Neoplásico
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Proteínas Serina-Treonina Quinases
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Proteínas de Ciclo Celular
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Análise de Sequência com Séries de Oligonucleotídeos
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Linhagem Celular Tumoral
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Genética
Limite:
Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2005
Tipo de documento:
Article