Serological and molecular study of three cases with a rare Bx02 blood group / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 65-67, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-345323
ABSTRACT
<p><b>OBJECTIVE</b>To determine the genotypes of three blood samples suspected as B subtype through DNA sequencing.</p><p><b>METHODS</b>The samples were first genotyped with PCR-SSP. Exons 6 and 7 of the ABO genes were subjected to PCR, direct sequencing, and cloned sequencing to determine the genotypes.</p><p><b>RESULTS</b>Serological results of the three samples were similar, with red cells being weakly agglutinatable by anti-B and serum containing anti-B. The samples were preliminarily genotyped as B/O1. Sequencing analysis showed that all three samples contained an O allele and a 905A>G mutation of the B gene, which was previously defined as Bx02.</p><p><b>CONCLUSION</b>Through sequencing analysis, the three samples typed as B subtype with serological testing were identified as Bx phenotype. The genotype of samples 1 and 2 was Bx02/O101, and that of sample 3 was Bx02/O102.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Sistema ABO de Grupos Sanguíneos
/
Tipagem e Reações Cruzadas Sanguíneas
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Éxons
/
Análise de Sequência de DNA
/
Alelos
/
Genética
/
Genótipo
Tipo de estudo:
Estudo prognóstico
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2017
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS