Application of CRISPR-Cas9 genome editing for constructing animal models of human diseases / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 559-563, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-345409
ABSTRACT
The CRISPR-Cas9 system is a new targeted nuclease for genome editing, which can directly introduce modifications at the targeted genomic locus. The system utilizes a short single guide RNA (sgRNA) to direct the endonuclease Cas9 in the genome. Upon targeting, Cas9 can generate DNA double-strand breaks (DSBs). As such DSBs are repaired by non-homologous end joining (NHEJ) or homology directed repair (HDR), therefore facilitates introduction of random or specific mutations, repair of endogenous mutations, or insertion of DNA elements. The system has been successfully used to generate gene targeted cell lines including those of human, animals and plants. This article reviews recent advances made in this rapidly evolving technique for the generation of animal models for human diseases.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Edição de RNA
/
Modelos Animais de Doenças
/
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
/
Genética
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2016
Tipo de documento:
Artigo
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