High cell density culture of an engineered yeast strain for sclareol production / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 147-151, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-345518
ABSTRACT
Cell growth profiles were evaluated in shake-flask culture to improve sclareol production by the engineered yeast strain Saccharomyces cerevisiae S7. Product formation was tightly coupled with cell growth. High cell density cultures were performed with different carbon sources using a dissolved oxygen level feedback-control strategy in a 3 L bioreactor. The titers of sclareol were 253 mg/L, 386 mg/L and 408 mg/L, respectively, when glucose, ethanol and glucose/ethanol mixture were used as the carbons sources. The maximal titer was 27-fold higher than that obtained under shake-flask culture conditions. The results suggested that the presence of ethanol was beneficial to sclareol production. These results provided useful information for optimization of yeast cell factory and efficient production of terpenoids.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oxigênio
/
Saccharomyces cerevisiae
/
Microbiologia Industrial
/
Reatores Biológicos
/
Meios de Cultura
/
Diterpenos
/
Etanol
/
Glucose
/
Metabolismo
/
Métodos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2015
Tipo de documento:
Artigo
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