Construction and characterization of an epitope-mutated Asia 1 type foot-and-mouth disease virus / 生物工程学报
Chinese Journal of Biotechnology
; (12): 96-104, 2015.
Article
em Zh
| WPRIM
| ID: wpr-345523
Biblioteca responsável:
WPRO
ABSTRACT
To generate an epitope-mutated foot-and-mouth disease virus (FMDV) as a marker vaccine, the infectious clone pAsia 1-FMDV containing the complete genomic cDNA of Asia 1 type FMDV was used as backbone, the residues at positions 27 and 31 in the 3D gene were mutated (H27Y and N31R). The resulting plasmid pAsia 1-FMDV-3DM encoding a mutated epitope was transfected into BHK-21 cells and the recombinant virus rAsia 1-3DM was rescued. The recombinant virus showed similar biological characteristics comparable with the parental virus. In serological neutralization test the antisera against recombine virus have a good reactivity with parental virus. The antisera against the mutant virus were shown to be reactive with the mutated epitope but not the wild-type one. The results indicated that the two virus strains could be distinguished by western blotting using synthetic peptides. This epitope-mutated FMDV strain will be evaluated as a potential marker vaccine against FMDV infections.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Testes de Neutralização
/
Transfecção
/
Linhagem Celular
/
DNA Complementar
/
Vírus da Febre Aftosa
/
Genética
/
Soros Imunes
/
Epitopos
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2015
Tipo de documento:
Article