Preparation and vitality detection of protoplast in Salvia miltiorrhiza Bunge / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1612-1621, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-345562
ABSTRACT
We prepared protoplasts from Salvia miltiorrhiza Bunge suspension culture cells. Then, the protoplasts' vitality and functions were tested by fluorescein diacetate staining method and Fluo-3/AM flourescent probe. The optimal condition of protoplast isolation was Cellulase R-10 1.5%, Pectinase Y-23 0.3%, Macerozyme R-10 0.5%, 40 r/min 12 h, 600 r/min 5 min, and the protoplasts yield was 1.1x10(6) cells/g FW, the vitality was more than 95% by using fluorescein diacetate staining method. It has been confirmed that calcium fluorescent probe Fluo-3/AM can be successfully loaded into protoplasts.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Protoplastos
/
Xantenos
/
Celulase
/
Química
/
Técnicas de Cultura de Células
/
Salvia miltiorrhiza
/
Corantes Fluorescentes
/
Compostos de Anilina
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2014
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS