Construction and characterization of a novel recombinant retroviral vector expressing mouse T-bet / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1586-1593, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-345565
ABSTRACT
In order to study T-bet function in mouse cells, a novel retroviral vector expressing mouse T-bet and reporter gene Thy1.1 was constructed. Retrovirus particles were then produced by transfection of the recombinant retroviral plasmid into a packaging cell line Platinum-E. The recombinant retrovirus played considerable infection ability. T-bet expression was then identified by FACS after infection of CD4+ primary T cells from T-bet knockout mouse with recombinant retrovirus. To determine if exogenous expressing T-bet has normal function, we checked the expression level of T-bet target gene, Ifng. IFN-y expression was upregulated in the T-bet knockout T cells infected with recombinant retrovirus. In conclusion, we successfully constructed an effective mouse T-bet recombinant retroviral vector.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Retroviridae
/
Proteínas Recombinantes
/
Linfócitos T
/
Transfecção
/
Linhagem Celular
/
Interferon gama
/
Camundongos Knockout
/
Proteínas com Domínio T
/
Vetores Genéticos
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2014
Tipo de documento:
Artigo
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