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Construction and characterization of a novel recombinant retroviral vector expressing mouse T-bet / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1586-1593, 2014.
Artigo em Chinês | WPRIM | ID: wpr-345565
ABSTRACT
In order to study T-bet function in mouse cells, a novel retroviral vector expressing mouse T-bet and reporter gene Thy1.1 was constructed. Retrovirus particles were then produced by transfection of the recombinant retroviral plasmid into a packaging cell line Platinum-E. The recombinant retrovirus played considerable infection ability. T-bet expression was then identified by FACS after infection of CD4+ primary T cells from T-bet knockout mouse with recombinant retrovirus. To determine if exogenous expressing T-bet has normal function, we checked the expression level of T-bet target gene, Ifng. IFN-y expression was upregulated in the T-bet knockout T cells infected with recombinant retrovirus. In conclusion, we successfully constructed an effective mouse T-bet recombinant retroviral vector.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Retroviridae / Proteínas Recombinantes / Linfócitos T / Transfecção / Linhagem Celular / Interferon gama / Camundongos Knockout / Proteínas com Domínio T / Vetores Genéticos / Metabolismo Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Retroviridae / Proteínas Recombinantes / Linfócitos T / Transfecção / Linhagem Celular / Interferon gama / Camundongos Knockout / Proteínas com Domínio T / Vetores Genéticos / Metabolismo Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2014 Tipo de documento: Artigo