Modification and decoration of transposase: a review / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1504-1514, 2014.
Article
em Zh
| WPRIM
| ID: wpr-345573
Biblioteca responsável:
WPRO
ABSTRACT
Transposons are the mobile and autonomic replication DNA fragments in genomes. With more understanding of the structure and function of transposons, numerous transposons have been developed to the genetics tool for gene function analysis, gene transformation and gene therapy. The low transpositional activity of the natural transposons is the main obstacles to the utilization of transposons. Recently, with the progress in bioinformatics and protein engineering methods, researchers have reconstructed and optimized natural transposases to create hyperactive transposases that catalyze the transposition with high efficiency. The resulted hyperactive transposons have been applied to gene-modification and gene-tagging. Meanwhile, transposase chimeras were created by protein fusion technology. The insertion characteristic of transposons were artificially regulated which could be utilized in gene therapy.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Elementos de DNA Transponíveis
/
Terapia Genética
/
Engenharia de Proteínas
/
Química
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Marcação de Genes
/
Transposases
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2014
Tipo de documento:
Article