Effect of salt on minor ampullate silk spidroin modules / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1308-1317, 2014.
Article
em Zh
| WPRIM
| ID: wpr-345593
Biblioteca responsável:
WPRO
ABSTRACT
To study the effect of physiological conditions on spidroins, we analyzed NTR1SR2CT module secondary structure, aggregation and silk-formation influenced by different salts (in different concentration intervals). According to the full-length Araneus ventricosus MiSp sequence, NTR1SR2CT module was constructed and expressed in Escherichia coli BL21 (DE3), and the recombinant proteins were purified by denaturation method mediated by 8 mol/L urea. Random coil and helix are the main secondary structures of NTR1SR2CT and could be induced into beta-sheet by drying natively and lyophilization, where methanol can be used as a promoter. Furthermore, potassium and phosphate cations can cause significant NTR1SR2CT protein aggregation and silk-formation. The results could be a basis for the determination of silk-formation mechanism, and also useful for industrialized generation of high performance spider silk-like fibers.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Sais
/
Aranhas
/
Química
/
Estrutura Secundária de Proteína
/
Fibroínas
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2014
Tipo de documento:
Article