Effect of IL-18BP on Fractalkine chemokine expression in the kidney tissue of rats with renal fibrosis / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 1134-1138, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-345629
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of Fractalkine (FKN) in the kidney tissue of rats with renal fibrosis and the effect of IL-18BP on FKN.</p><p><b>METHODS</b>Male Wister rats were randomly assigned to sham-operation (n=24), unilatral ureteral obstruction (UUO, n=22), and IL-18 binding protein (IL-18BP) treatment groups (n=23). The UUO model was prepared by unilateral ureteral ligation in the later two groups. The IL-18BP treatment group received an intraperitoneal injection of IL-18BP (0.1 mg/kg) every other day after UUO inducement, for 7 times, while normal saline was administered in the other two groups. Seven or eight rats of every group were sacrificed at 3, 7 or 14 days after IL-18BP or normal saline injections. FKN levels at various times were detected by immunohistochemistry and Western blot.</p><p><b>RESULTS</b>Compared with the sham-operation group, FKN levels in the kidney tissue of the untreated UUO group increased significantly at all time points (P<0.01). IL-18BP treatment decreased significantly FKN levels in the kidney tissue at all time points compared with the untreated UUO group (P<0.01).</p><p><b>CONCLUSIONS</b>IL-18BP treatment may down-regulate the increased FKN levels of the rat kidney tissue caused by UUO, possibly thus delays the occurrence and development of renal fibrosis.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Obstrução Ureteral
/
Fibrose
/
Western Blotting
/
Ratos Wistar
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Alergia e Imunologia
/
Quimiocina CX3CL1
/
Genética
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2013
Tipo de documento:
Artigo
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