Evaluation of VITEK Mass Spectrometry (MS), a Matrix-Assisted Laser Desorption Ionization Time-of-Flight MS System for Identification of Anaerobic Bacteria
Annals of Laboratory Medicine
;
: 69-75, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-34573
ABSTRACT
BACKGROUND:
By conventional methods, the identification of anaerobic bacteria is more time consuming and requires more expertise than the identification of aerobic bacteria. Although the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are relatively less studied, they have been reported to be a promising method for the identification of anaerobes. We evaluated the performance of the VITEK MS in vitro diagnostic (IVD; 1.1 database; bioMerieux, France) in the identification of anaerobes.METHODS:
We used 274 anaerobic bacteria isolated from various clinical specimens. The results for the identification of the bacteria by VITEK MS were compared to those obtained by phenotypic methods and 16S rRNA gene sequencing.RESULTS:
Among the 249 isolates included in the IVD database, the VITEK MS correctly identified 209 (83.9%) isolates to the species level and an additional 18 (7.2%) at the genus level. In particular, the VITEK MS correctly identified clinically relevant and frequently isolated anaerobic bacteria to the species level. The remaining 22 isolates (8.8%) were either not identified or misidentified. The VITEK MS could not identify the 25 isolates absent from the IVD database to the species level.CONCLUSIONS:
The VITEK MS showed reliable identifications for clinically relevant anaerobic bacteria.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bactérias Anaeróbias
/
Líquidos Corporais
/
RNA Ribossômico 16S
/
Técnicas de Tipagem Bacteriana
/
Análise de Sequência de DNA
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Bases de Dados Genéticas
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Annals of Laboratory Medicine
Ano de publicação:
2015
Tipo de documento:
Artigo
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