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Effect of EBV immediate-early protein Zta on the cell cycle of Daudi cells and its mechanisms / 中华血液学杂志
Chinese Journal of Hematology ; (12): 47-50, 2012.
Artigo em Chinês | WPRIM | ID: wpr-345947
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of EBV immediate-early protein Zta on cell cycle of Daudi cells and the involved mechanisms.</p><p><b>METHODS</b>The expression vector encoding Zta was constructed and electroporated into Daudi cells. Flow cytometric analysis was used to detect the cell cycle, Western blot to the protein levels of p21, Rb and E2F-1.</p><p><b>RESULTS</b>The vector was constructed successfully, the expression of Zta protein inhibited the proliferation of Daudi cells and promoted cell cycle from G(0)/G(1) phase \[(30.0 ± 3.4)%\] to S phase \[(47.7 ± 1.1)%\]. Meanwhile, Rb expression was significantly downregulated, E2F-1 and p21 expression upregulated by Zta.</p><p><b>CONCLUSION</b>Zta could promote G(0)/G(1) phase to S phase transition in Daudi cells, which might be associated with the reduced expression of Rb and increased expression of E2F-1 and p21 protein.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas Virais / Ciclo Celular / Ativação Transcricional / Transativadores / Divisão Celular / Proteína do Retinoblastoma / Proteínas Imediatamente Precoces / Herpesvirus Humano 4 / Linhagem Celular Tumoral / Inibidor de Quinase Dependente de Ciclina p21 Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Hematology Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas Virais / Ciclo Celular / Ativação Transcricional / Transativadores / Divisão Celular / Proteína do Retinoblastoma / Proteínas Imediatamente Precoces / Herpesvirus Humano 4 / Linhagem Celular Tumoral / Inibidor de Quinase Dependente de Ciclina p21 Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Hematology Ano de publicação: 2012 Tipo de documento: Artigo