Research on mesenchymal stem cells image segmentation based on morphology / 生物医学工程学杂志

ABSTRACT

A kind of research on mesenchymal stem cells (MSCs) segmentation is reported in this paper. The research of its kind has become more and more popular recently. Top-bottom hat algorithm of gray morphology is applied to image preprocessing. The radius of the ball of the structure function is 30 in the algorithm. After preprocessing, binary image is gained cursoryily through distinguishing foreground from background using two thresholds. Noise is removed by applying morphology algorithm. It is easy to differentiate foreground from background in the result image. Finally, area and number of cells can be calculated respectively.