Identification of phosphoproteome in mice neurons by isotope-labeling technique combining with chemiluminescence Western blotting method / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 898-901, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-346046
ABSTRACT
This study was aimed to work out a simple, applicable, sensitive and specific protocol for the identification of phosphoproteome. Isotope-labeling, two-dimensional electrophoresis, autoradiography and so on were used to establish a phosphoproteome map of mice neurons, and then chemiluminescence Western blotting was utilized to detect three phosphoproteins PI3Kr3, MEK1 and PKCalpha selectively. The results of comparison showed that the blots of PI3Kr3, MEK1 and PKCalpha on autoradiography map were almost identical with the blots of PI3Kr3, MEK1 and PKCalpha on chemiluminescence Western blotting maps. So this protocol based on isotope labeling and chemiluminescence Western blotting methods has proven to be sensitive and specific in the identification of phosphoproteome.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfoproteínas
/
Radioisótopos de Fósforo
/
Encéfalo
/
Eletroforese em Gel Bidimensional
/
Células Cultivadas
/
Química
/
Western Blotting
/
Sensibilidade e Especificidade
/
Proteoma
/
Biologia Celular
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2007
Tipo de documento:
Artigo
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