Detection of tobacco mosaic virus (TMV) in Rehmannia glutinosa f. hueichingensis by IC-RT-PCR / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2092-2095, 2013.
Article
em Zh
| WPRIM
| ID: wpr-346436
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a rapid, sensitive and efficient detection method for tobacco mosaic virus (TMV), and provide technical support of TMV detection of Rehmannia glutinosa f. hueichingensis. The virus-free plantlets could be produced on a large scale to ameliorate breed degeneration caused by viral disease.</p><p><b>METHOD</b>Specific primers were designed based on the conserved region of coat protein(CP) gene of TMV. Immunocapture RT-PCR (IC-RT-PCR) was employed to detect TMV and the sequence of the products was detected.</p><p><b>RESULT</b>The expected nucleotide acid fragments were amplified by IC-RT-PCR. The homology of nucleotide acid sequence and amino acid sequence were 95.29% and 96.7% between the PCR products and the CP gene of TMV (accession number AY555269).</p><p><b>CONCLUSION</b>The method was established for the detection of TMV in R. glutinosa f. hueichingensis by IC-RT-PCR. This detection combined molecular biology technology with immunology, was convenient for a quick, sensitive and simple detection of TMV.</p>
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Índice:
WPRIM
Assunto principal:
Vírus do Mosaico do Tabaco
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Virologia
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Dados de Sequência Molecular
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Sequência de Bases
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Sequência de Aminoácidos
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Rehmannia
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Alergia e Imunologia
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Genética
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Métodos
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2013
Tipo de documento:
Article