Effect of pulchinenoside in regulating FLS SFRP2 expression of RA model rats / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1977-1981, 2013.
Article
em Zh
| WPRIM
| ID: wpr-346460
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of pulchinenoside (PULC) in modulating SFRP2 expression in fibroblast-like synoviocytes (FLS) of rheumatoid arthritis (RA) model rats.</p><p><b>METHOD</b>The effect of PULC in treating RA rats was evaluated by rat arthritis score and paw swelling score. The inhibitory effect of PULC on FLS proliferation was detected by MTT reagent. The effects of PULC gavage treatment in modulating gene expression of FLS SFRP2, critical gene beta-catenin of Wnt pathway and downstream effector genes C-myc of of Wnt pathway were detected by RT-PCR and Western blotting.</p><p><b>RESULT</b>PULC had a significant effect in treating RA rats and that SFRP2 expression was down-regulated in FLS. After PULC gavage treatment, FLS SFRP2 expression was obviously up-regulated, whereas beta-catenin and C-myc gene expressions were significantly down-regulated.</p><p><b>CONCLUSION</b>PULC can inhibit abnormal proliferation of synovial membrane by modulating Wnt pathway of RA rats.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
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Artrite Reumatoide
/
Saponinas
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Membrana Sinovial
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Regulação da Expressão Gênica
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Ratos Sprague-Dawley
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Modelos Animais de Doenças
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Tratamento Farmacológico
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Genética
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Proteínas de Membrana
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2013
Tipo de documento:
Article