A study on relationship between rat brain tissues hurts of the gas explosion and the expression of protein kinase CamRNA and c-fos gene / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 24-26, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-346597
ABSTRACT
<p><b>OBJECTIVES</b>To explore relationship between rat brain tissues hurts of gas explosion and the expression of Protein Kinase C alpha mRNA.</p><p><b>METHODS</b>Build up rat hurt model of gas explosion. In Situ Hybridization (IDH) technique was used to test Protein Kinase C alpha mRNA. Immunohistochemical Assays (IHA) was used to determine c-fos gene protein.</p><p><b>RESULTS</b>Only a little a mount expression of PKC alpha mRNA and c-fos of the control group was detected. The expression of the cerebral cortex and hippocampus of PKC alpha mRNA 24 h, 48 h and the 48 h increased obviously, and the 48 h reached the peak of expression; (t = 4.12 P < 0.01). The expression of c-fos protein of the cerebral cortex and hippocampus started to increase obviously at 0.5 h and the 4 h reached the peak, then the strength lowered gradually and the expression level came back normal level on fifth day.</p><p><b>CONCLUSION</b>The anoxia of brain tissues due to the gas explosion may promote the expression of PKCamRNA, and PKCamRNA could regulate the expression of the gene of c-fos. Both PKCamRNA and the gene of c-fos are involved in harmful processes to the nerve cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Gases Lacrimogênios
/
Encéfalo
/
Lesões Encefálicas
/
RNA Mensageiro
/
Proteínas Proto-Oncogênicas c-fos
/
Ratos Sprague-Dawley
/
Modelos Animais de Doenças
/
Proteína Quinase C-alfa
/
Explosões
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2005
Tipo de documento:
Artigo
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