Adipose tissue-derived stromal cells express neuronal phenotypes / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 425-429, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-346658
ABSTRACT
<p><b>BACKGROUND</b>Adipose tissue-derived stromal cells (ADSCs) can be greatly expanded in vitro, and induced to differentiate into multiple mesenchymal cell types, including osteogenic, chondrogenic, myogenic, and adipogenic cells. This study was designed to investigate the possibility of ADSCs differentiating into neurons.</p><p><b>METHODS</b>Adipose tissue from rats was digested with collagenase, and adherent stromal cells were cultured. A medium containing a low concentration of fetal bovine serum was adopted to induce the cells to differentiate. ADSCs were identified by immunocytochemistry, and semi-quantitative RT-PCR was applied to detect mRNA expression of neurofilament 1 (NF1), nestin, and neuron-specific enolase (NSE).</p><p><b>RESULTS</b>Nestin-positive cells were found occasionally among ADSCs. ADSCs were found to express NSE mRNA and nestin mRNA, but not NF1 mRNA. ADSCs could differentiate into neuron-like cells in a medium composed of a low concentration of fetal bovine serum, and these differentiated cells displayed complicated neuron-like morphologies.</p><p><b>CONCLUSIONS</b>The data support the hypothesis that adipose tissue contains stem cells capable of differentiating into neurons. These stem cells can overcome their mesenchymal commitment, and may represent an alternative autologous stem cell source for CNS cell transplantation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Fosfopiruvato Hidratase
/
Fisiologia
/
Imuno-Histoquímica
/
Diferenciação Celular
/
Células Cultivadas
/
Tecido Adiposo
/
Proteínas de Neurofilamentos
/
Biologia Celular
/
Nestina
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2004
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS