The preparation of nano-hydroxyapatite and preliminary observation on its effects on the occlusion of dentinal tubule / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To synthesize nano-hydroxyapatite (nano-HA) and evaluate its effect on the occlusion of dentinal tubules in vitro, and therefore provide evidence for the clinical application.</p><p><b>METHODS</b>(NH(4))(2)HPO(4) and Ca(NO3)2 were used to form nano-HA, which was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectrometer (FTIR) and X-ray diffractometer (XRD) respectively. Twenty-four dentin slabs were obtained from 8 healthy third molars and randomly divided into 3 groups, which were control group, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) group, and nano-HA group. After the CPP-ACP and nano-HA were topically applied to the slabs of two study groups twice a day for 7 days, the surface of slab dentin was observed using SEM.</p><p><b>RESULTS</b>SEM, TEM, FTIR, and XRD tests showed that nano-HA was synthesized successfully. SEM observations revealed that the sealing of dentinal tubules of nano-HA group was extremely high when compared with those of control and CPP-ACP group.</p><p><b>CONCLUSIONS</b>In comparison with CPP-ACP, nano-HA could occlude dentinal tubules more effectively in vitro.</p>