Immunofluorescence examination of CK-13 expression in cell line KB differentiated by all-trans retinoic acid or As2 O3 / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 53-55, 2009.
Article
em Zh
| WPRIM
| ID: wpr-346773
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine the expression of cytokeratin-13 (CK-13) in oral squamous cell carcinoma (OSCC) and to discuss the effects of all-trans retinoic acid (ATRA) or arsenic trioxide (As2 O3) on the differentiation of human oral undifferentiated squamous cell carcinoma cell line KB cells.</p><p><b>METHODS</b>The cultured KB cells were divided into three groups, ATRA group, As2 O3 group, and control. The expression of CK-13 in KB cells was detected using the immunofluorescence before and after KB cells were induced by ATRA or As2 O3.</p><p><b>RESULTS</b>The expression rates of CK-13 in KB cells in the ATRA group and As2 O3 group were significantly higher than that in the control (P < 0.05), but there was no significant difference in the expression between ATRA and As2 O3 group(P > 0.05).</p><p><b>CONCLUSIONS</b>ATRA and As2 O3 both have the ability to differentiate the KB cells, and the expression is associated with the degree of tumor differentiation. CK-13 may serve as a molecular marker to evaluate the effect of the differentiation treatment on OSCC.</p>
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Índice:
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Assunto principal:
Óxidos
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Farmacologia
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Arsenicais
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Tretinoína
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Neoplasias Bucais
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Células KB
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Carcinoma de Células Escamosas
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Diferenciação Celular
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Imunofluorescência
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Queratina-13
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2009
Tipo de documento:
Article