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In vitro analysis of intake of minocycline by mature rat mandibular osteoblasts / 中华口腔医学杂志
Chinese Journal of Stomatology ; (12): 28-31, 2009.
Artigo em Chinês | WPRIM | ID: wpr-346780
ABSTRACT
<p><b>OBJECTIVE</b>To observe the intake of minocycline and its amount in mature rat mandibular osteoblasts (MRMOB) in vitro, and to identify the feasibility of intracellular anti-bacterial activity of minocycline.</p><p><b>METHODS</b>Four groups of MRMOB were incubated in 100 mg/L minocycline for 15, 30, 45 and 60 minutes respectively, and the accumulation of minocycline within MRMOB was measured using a fluorescence spectrophotometer.</p><p><b>RESULTS</b>The intracellular accumulation amount of minocycline in the four groups of MRMOB was (17.29 +/- 1.49), (16.87 +/- 1.57), (16.96 +/- 1.67) and (17.94 +/- 1.63) mg/g respectively after osteoblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) which contained minocycline for 15, 30, 45 and 60 min. There was no significant difference in amount of minocycline among the four groups of MRMOB.</p><p><b>CONCLUSIONS</b>The mature rat mandibular osteoblasts can ingest minocycline, and the accumulation amount of minocycline in MRMOB is irrelevant with the exposure time of MRMOB to minocycline.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Osteoblastos / Farmacocinética / Células Cultivadas / Ratos Sprague-Dawley / Biologia Celular / Mandíbula / Metabolismo / Minociclina Limite: Animais Idioma: Chinês Revista: Chinese Journal of Stomatology Ano de publicação: 2009 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Osteoblastos / Farmacocinética / Células Cultivadas / Ratos Sprague-Dawley / Biologia Celular / Mandíbula / Metabolismo / Minociclina Limite: Animais Idioma: Chinês Revista: Chinese Journal of Stomatology Ano de publicação: 2009 Tipo de documento: Artigo