Molecular identification of astragali radix and its adulterants by ITS sequences / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3773-3776, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-346840
ABSTRACT
<p><b>OBJECTIVE</b>To explore a new method for identification Astragali Radix from its adulterants by using ITS sequence.</p><p><b>METHOD</b>Thirteen samples of the different Astragali Radix materials and 6 samples of the adulterants of the roots of Hedysarum polybotrys, Medicago sativa and Althaea rosea were collected. ITS sequence was amplified by PCR and sequenced unidirectionally. The interspecific K-2-P distances of Astragali Radix and its adulterants were calculated, and NJ tree and UPGMA tree were constructed by MEGA 4.</p><p><b>RESULT</b>ITS sequences were obtained from 19 samples respectively, there were Astragali Radix 646-650 bp, H. polybotrys 664 bp, Medicago sativa 659 bp, Althaea rosea 728 bp, which were registered in the GenBank. Phylogeny trees reconstruction using NJ and UPGMA analysis based on ITS nucleotide sequences can effectively distinguish Astragali Radix from adulterants.</p><p><b>CONCLUSION</b>ITS sequence can be used to identify Astragali Radix from its adulterants successfully and is an efficient molecular marker for authentication of Astragali Radix and its adulterants.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Especificidade da Espécie
/
DNA Ribossômico
/
RNA Ribossômico
/
Dados de Sequência Molecular
/
Química
/
Classificação
/
Análise de Sequência de DNA
/
Raízes de Plantas
/
DNA de Plantas
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Artigo
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